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Recurrent miscarriage associated with half as much vitamin D getting to fetus – Sept 2016

Decreased expression of the vitamin D receptor in women with recurrent pregnancy loss

Archives of Biochemistry and Biophysics, Volume 606, 15 September 2016, Pages 128–133, doi:10.1016/j.abb.2016.07.021
Xiaoting Yana, Liqin Wangb, Chunfang Yanc, Xinwen Zhanga, c, Lingyun Huid, Qiu Shenga, Mingzhan Xuee, Xuewen Yua, yuxuewen2007 at 163.com

Vitamin D Life Comment

Vitamin D blood in the blood does not equate to vitamin D in the cells
In this case a 50% reduction in Vitamin D Receptor might be offset by having 2X higher vitamin D blood level
Thus, a woman with recurrent pregnancy loss may need to have a blood level 2 times higher


The items in Pregnancy and Vitamin D Receptor are listed here:

Highlights
• VDR is expressed in the fetal-maternal interface in first trimester of pregnancy.
• Women with RPL have a lower VDR expression in villous and decidual tissues.
• There are lower VDR levels in villous cytotrophoblasts and stromal cells in RPL.
• There are lower VDR levels in decidual glandular epithelial and stromal cells in RPL.

The multiple functions of vitamin D3 have stimulated interest in the role that this vitamin may play during pregnancy. The present study investigated the expression of the vitamin D receptor (VDR) in women during the first trimester of pregnancy in order to determine whether VDR is associated with recurrent pregnancy loss (RPL).
Forty women at 7–10 weeks gestation with RPL and 40 women of similar gestational age with a healthy pregnancy were recruited. VDR mRNA and protein in chorionic villi and decidua were evaluated by immunohistochemistry, confocal laser scanning microscopy (CLSM), western blot, and quantitative real-time polymerase chain reaction. The serum levels of VDR were measured by an enzyme-linked immunosorbent assay.
Women with RPL had a significantly weaker expression of VDR mRNA in villi and decidual tissues compared with the control women (both p < 0.0001). Western blot analysis showed an approximately 46% decrease in VDR expression in villi and a 52% decrease in decidua in the RPL vs. the controls.
Serum VDR levels were also significantly lower in the RPL group than in the control group (p = 0.003). Compared with the controls, immunohistochemical and CLSM analysis revealed significantly lower VDR expression in villous cytotrophoblasts and stromal cells, as well as in decidual glandular epithelial and stromal cells (all p < 0.05). In conclusion, these observations show that women with RPL have lower levels of VDR expression in chorionic villi, decidua and serum compared with normal pregnant women, suggesting that decreased VDR expression in the first trimester pregnancy may be associated with RPL.

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