Inhaling Vitamin D nanoemulsion through nose gets lots more to the brain (mice) – July 2020

Enhancement in brain uptake of Vitamin D3 nanoemulsion for treatment of cerebral ischemia: Formulation, Gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models

Journal of Microencapsulation https://doi.org/10.1080/02652048.2020.1801870
Mukesh Kumar,Dhruv Kumar Nishad,Anoop kumar,Aseem Bhatnagar,Ritu Karwasra,Kushagra Khanna, show all

The study used radioactive Vitamin D

 Download the PDF from sci-hub via Vitamin D Life

Aim: For the treatment of cerebral ischemia, Vitamin-D3 loaded nanoemulsion were developed.

Method: Tween 20 and polyethylene glycol were choosen as surfactant/co-surfactant, while oleic acid as oil phase. The formulation was characterized for various in-vitro parameters. Targeting efficiency was investigated through radiometry, gamma scintigraphy and efficacy was studied in transient middle cerebral artery occlusion (MCAo) rat model.

Result: Vitamin D3-Nanoemulsion showed a mean size range of 49.29 ± 10.28 nm with polydispersity index 0.17 ± 0.04 and zeta potential 13.77mV. Formulation was found stable during thermodynamic stability study and permeated within 180 min through sheep nasal mucosa (permeation coefficient 7.873 ± 0.884 cm/h). Gamma scintigraphy and radiometry assay confirmed better percentage deposition (2.53 ± 0.17%) of 99mTc-Vitamin D3-Nanoemulsion through nasal route compared to IV administered 99mTc-Vitamin D3 solution (0.79 ± 0.03%). Magnetic Resonance Imaging (MRI) of the ischemic model confirmed better efficacy of Vitamin D3-Nanoemulsion.

Conclusion: This work demonstrated better permeation, deposition and efficacy of VitaminD3-Nanoemulsion through intranasal route.

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